Journal: Bioactive Materials

Article Title: Engineered atopic dermatitis models for recreating hypoxic conditions in atopic dermatitis microenvironments

doi: 10.1016/j.bioactmat.2025.12.045

Figure Lengend Snippet: Neuroregulatory role of AD-specific COL6A5+ FBs. (a) Heatmap of summarized GO terms enriched in COL6A5+ FBs. (b) Heatmap of summarized GO terms overrepresented in selected regulons responsible for neuro-regulation (TCF4_extended and TFAP2C_extended). (c) Inferred LR pairs mediating interaction between COL6A5+ FBs and sensory neurons in the skin. (d) Dot plot of the POSTN expression in FB subtypes.

Article Snippet: Primers for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), HIF-1α, periostin (POSTN), tenascin-C (TNC), Wnt family member 5 A (WNT5A), and COL6A5 were supplied by Cosmogenetech (Seongdong-gu, Seoul).

Techniques: Expressing

Journal: Bioactive Materials

Article Title: Engineered atopic dermatitis models for recreating hypoxic conditions in atopic dermatitis microenvironments

doi: 10.1016/j.bioactmat.2025.12.045

Figure Lengend Snippet: Analysis of periostin expression. (a) Schematic representations of periostin expression analysis. Gene expression under (b) 21 % p O 2 condition and (c) 5 % p O 2 condition (qRT-PCR). (d) Immunofluorescence staining of periostin. (e) Protein expression (ELISA) analysis. (f) Volcano plot comparing FBs under hypoxia without IL-4 (down – blue) and hypoxia with IL-4 (up – red). Labeled genes are those known to be associated with AD pathogenesis and meet the DEG criteria (padj < 0.05 & log2FC > 0). (g) Normalized expression heatmap of selected genes associated with AD pathogenesis. Data are presented as the mean ± s.d. (b, c, e) (n = 3–5). Statistical significance and p values are determined by one-way ANOVA with Tukey's post-hoc comparison (b, c) and unpaired two-tailed Student's t-test (e). (∗) indicates a significant difference (∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001). (N.D.; Not detected).

Article Snippet: Primers for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), HIF-1α, periostin (POSTN), tenascin-C (TNC), Wnt family member 5 A (WNT5A), and COL6A5 were supplied by Cosmogenetech (Seongdong-gu, Seoul).

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Labeling, Comparison, Two Tailed Test

Journal: The World Journal of Men's Health

Article Title: Comprehensive Analysis of N6-Methyladenosine Modification Profiling in Diabetic Erectile Dysfunction

doi: 10.5534/wjmh.240328

Figure Lengend Snippet: Subpopulation distribution of hub genes. (A) Clustering visualization of subpopulations in the corpus cavernosum. (B) Expression distribution of hub genes in different cells of corpus cavernosum. The expression distribution of POSTN (C, D) and LOX (E, F) in the tissue of corpus cavernosum. UMAP: uniform manifold approximation and projection, EC: endothelial cell, FB: fibroblast, SMC: smooth muscle cell, SWC: Schwann cell, MAC: macrophage, T: T cell.

Article Snippet: Similarly, the sections of corpus cavernosum were subjected to immunohistochemistry to detect the protein expression of POSTN (66491-1-Ig, Proteintech) and LOX (A11504, ABclonal).

Techniques: Expressing